Exploring the antimicrobial potential of biologically synthesized zero valent iron nanoparticles

Abstract The widespread use of antibiotics has resulted in the emergence multidrug-resistant bacteria. Therefore, it is essential to explore alternative strategies effectively combat medically significant resistant pathogens. In recent years, nanoparticles (NPs) have emerged as a promising source antimicrobial agents. While nanoscale particles were traditionally synthesized using chemical techniques, development metallic NPs biological methods garnered attention. This current study focuses on synthesis iron (Fe NPs) metal-tolerant fungal strains, numerous microorganisms serve environmentally safe and durable precursors produce persistent bi-functional NPs. involved isolation evaluation ten strains that are heavy metals determine their ability Fe biologically characterized X-ray diffraction (XRD), energy dispersive spectroscopy (EDX), scanning electron microscopy techniques. XRD results indicated presence nanopowder form, displaying series reflection angles (2 θ ) at 65° 75° indicating existence cubic planes. EDX analysis revealed ferrous ferric elements, along with zero-valent Micrographs surface topology displayed spherical aggregation Furthermore, exhibited antibacterial potential against selected bacterial including Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Cronobacter sakazakii, Listeria innocua, Enterococcus fecalis . demonstrates safe, produced through mycological means could be utilized antibiotic-resistant pathogenic strains.